口■■■▣口口②方法一：纯合宽叶3号染色体单体植株与窄叶染色体正常植株作为亲本杂交获得E,若相关基因不在3号染色体上，则应表示为EE×ee,后代都为E®：若相关基因在3号染色体上，则应表示为EO(O表示3号染色体缺失导致的无另一个E基因)，后代E:eO=1:1。方法二：纯合宽叶3号染色体单体植株与窄叶3号染色体单体植株作为亲本杂交获得F1,若相关基因不在3号染色体上，则应表示为EE×ee,后代都为Ee:若相关基因在3号染色体上，则应表示为E0(O表示3号染色体缺失导致的无另一个E基因)×eO,后代Ee:EO:e0:OO=1:1:1:l,一对同源染色体都缺失不能存活，因此宽叶：窄叶=2：1。20.(除特殊标注外，每空1分，共11分)(1)PCR3引物A和引物B引物A和引物D(2)引物B和引物C存在碱基互补配对片段，置于同一反应系统时它们会发生结合而失去作用(2分)2(2分)》(3)5'氨苄青霉素四环素氨苄青霉素【解析】(1)PCR3时，杂交DNA的两条链可以互为模板和引物，不需要引物。根据图中PCR1扩增的蛋白A基因部分，推出该过程需要的引物是引物A和引物B。由于PCR4扩增的是新的蛋白A基因，推出该过程需要的引物是引物A和引物D。(2)因为引物B和引物C为互补序列，在一个反应系统中同时进行PCR1和PCR2时，引物B和引物C会因发生结合而失去作用。将PCR1和PCR2得到的产物混合、变性后杂交，除能得到原本的PCR1和PCR2产物外，还有533一5'和35+5一3'两种新类型的杂交DNA分子。(3)因为引入的限制酶识别序列在模板链上没有对应的互补序列，所以应在引物的5'端引入，不影响3′端子链的延伸。因为重组质粒中的四环素抗性基因被破坏，所以在含氨苄青霉素的固体培养基上含质粒和含重组质粒的大肠杆菌能存活，而在含四环素的培养基上仅含质粒的大肠杆菌能存活。应先使用无菌的绒毡布压在含氨苄青霉素的固体培养基的菌落上，带出含质粒的大肠杆菌和含重组质粒的大肠杆菌，再移并压在含有四环素的固体培养基上，最终通过对比在含氨苄青霉素的固体培养基上获得含重组质粒的菌落。生物参考答案·第4页（共4页）

hefreusaid."igent technoloesoaeproblem faced by theconsuction industry,freeing workers70 their heavy abor.61.62.63.64.6765.66.68.69.70.第四部分写作（共两节，满分35分）第一节短文改错（共10小题，每小题1分，满分10分）假定英语课上老师要求同桌之间交换修改作文，请你修改你同桌写的以下作文。文中共有10处语言错误，每句中最多有两处。每处错误仅涉及一个单词的增加、刷除或修改。增加：在缺词处加一个漏字符号（八），并在其下面写出该加的词。刷除：把多余的词用斜线（八）划掉。修改：在错的词下画一横线，并在该词下面写出修改后的词。注意：1.每处错误及其修改均仅限一词；2.只允许修改10处，多者（从第11处起）不计分。Mr.Zhang is one of my favorite high school teacher.He is a very patient teacher,who often ex-plains us everything carefully to make us understood.He's also such kind and friendly that we alwaysturn to him for help.Deal with students can be annoying sometimes,but he never complained.Besides,he always explores new ways to make his lessons attractively.Above all,Mr.Zhang is constantly encour-aged us to get involved in classroom activities.He makes me to realize teaching is a very meaningful pro-fession.That's because I'm determined to be a teacher like him in the future.第二节书面表达（满分25分）假定你是李华，你的英国朋友Peter对中国诗词非常感兴趣并在他所在的学校加入了中国诗词俱乐部，现在因为太难学而打算放弃。请你发一封电子邮件鼓励他，内容包括：1.你的目的；2.你的建议。注意：1.词数100词左右。2.可适当增加情节，以使行文连贯。Dear Peter,Yours,Y高三模拟卷（八）·英语·A区专用：第8页（共八页）】Li Hua

B.The traditional dances in RwandaC.The importance of cows in Rwanda.D.The agricultural development of Rwanda.31.What is the main purpose of people's saying"nguhaye inka"in RwandaA.To wish someone well.B.To convey deep thanks.C.To congratulate someone.D.To respond to someone's question.DHave you ever made eye contact with a robot?It can be a very strange experience.Scien-tists even have a name for the feeling-the mysterious valley..Now,researchers in Italy have found that it is more than just a feeling.They ran an ex-periment that showed how a robot's gaze (can trick people into thinking they are social-ly interacting with a human being.That experience can slow a person's ability to makedecisions."Gaze is an extremely important social signal that we employ on a day-to-day basiswhen interacting with others,"said Professor Agnieszka Wykowska."The question is wheth-er the robot's gaze will cause very similar mechanisms in the human brain as another human'sgaze would,"Wykowska told Reuters.The team asked 40 people to play a video game,chicken.In the game,each player has todeeide whether to permit a car to drive straight toward another car or to turn to avoid a crash.The people were playing against a human-like robot sitting across from them.During breaks in the game,players had to look at the robot.Sometimes the robot wouldlook back and other times it would look away.As the interactions happened,the scientistscollected data on behavior and brain activity."Our results show that,actually,the humanbrain processes the robot's gaze as a social signal,and that signal has an impact on the waywe're making decisions,on the strategies we use in the game and also on our responses,"Wykowska said."The gaze of the robot affected decisions by delaying them,so humans weremuch slower in making the decisions in the game,"she added.The findings could be useful in helping to decide where and how human-like robots mightbe placed in the future.When we understand when and how robots change social behavior,wecan decide which sort of context is beneficial to humans and in which context this should notoccur.32.What does the experiment show in paragraph 2?A.Scientists always name the robots.B.A robot's gaze can mislead humans.C.Robots have the ability to make decisions.D.Robots like interacting with human beings.33.What did the team require participants to do?A.Learn to drive a car.B.Get rid of a car crash.·22-12-291C·【高三英语第6页（共10页）】

第33期参考答案第2版CHECKING CORNERI.1.bury2.destroy3.strike①destroyed②bury③struck.A 1.actually2.narrow3.rescue4.trapped5.injuredB 1.rely on2.teenage3.made a huge difference4.admired5.as if6.positive7.giving upⅢ.A 1.challenge2.extremely3.means4.lose heart5.make it6.attitude7.organizeB 1.impressions2.accommodation3.lectures4.castle5.looking forward toC 1.volcanoes2.amazing3.destination4.admire /appreciate5.No matter where they are第3版CHECKING CORNERI.1.have you 2.isn't it'3.can they4.didn't II.②There be.③SV④SP⑤S VIO DO⑥SVO⑦SVOA⑧SVOCI.NP:①②⑤⑦AdjP:④⑨AdvP:③⑥⑧IV.1.is coming2.doesn't leave3.doesn't arrive·4.are staying5.are spending6.are havingV.1.that/which2.that/who3.that /which4.that who5.that /who6.which7.that /whichVI.1-6 ECBAFD第4版PROGRESS TESTI.1-4 BBCA5-8 ACBAII.1-5 DEGBF

hats the author's attitude towards Xu Feihu and Pan Jianwei's trial?A.Ambiguous.34.What may be talked about in the following paragraph?B.Indifferent.C.Doubtful.DFavorable.A.Performing the trick over a long distance.B.Showing hidden objeetsnmngoC.Capturing the correct experimental data.AD.Applying the technology into hostage-taking.35.What's the best title for the passage?ASeeing hidden objects through wallsB.Engineers'task inside a locked roomC.NASA's interest in a new technologyD.Putting military applications into practice第二节(共5小题；每小题2分，满分10分)根据短文内容，从短文后的七个选项中选出能填入空白处的最佳选项。选项中有两项为多余选项。Do we NEED sleep to form long-term memories?When someone tells you to "sleep on it",they're usually suggesting that you avoidmaking improper decisions.36.DNow,pmight sm inonveninwhen yo'anxious about an xam But don'orry.Becuhw ththkrtouchingoveumembersng those things a week ater.37That suggests that long-term memocan be laid down without having to lay down.tht sleeplepyou rmmer things you've red.Decadesofstueshown that sleep helps totransform or develop recent experiences into lasting memorereidn o)nt ongmemory during efalnes.Sa,sepdepnvedntirvdelseansileansluftate betert remembring therignl becthan their fully rested fellows,but only when the item was presented in a new location.Ratsgotemloof the m objeets when they appeared out of context.3ofkdbyshethomemory consolidatiomkerhin their proper place.Nevertheless,scientistsare still convinced that sleep leads to the more effective,overall moreeffective,type of mmyWhen youare not fully rested,it is in generalmore difficulr tove n kind of memory in comparison when you are fully rested.A.Plus,sleep gives your brain a chance to restSomerimes they performed better than tats who slept高三英语第7页（共10面

综合能力评估试题(4)参考答案及部分解析参考答案1-5 CBBAA6-10 BCABC11-15 ACACB 16-20 BCACB21-25 ACADC26-30 BACAD31-35 ABDAC36-40 CGFBA41-45 DABDA 46-50 CABDC51-55 ADBDC56.than57.came58.the 559.to60.following 61.is62.excited63.me64.times65.more beautiful写作第一节One possible version:Dear BobI'm Li Hua from the Street Dance Club of our school.I'm writing to warmly invite you to join theclubFounded in 2010,the Street Dance Club has become one of the most popular clubs in our school.With 40 members,including 3 exchange students,the club provides a great place for studentsto learn and dance.We hold activities regularly,from School Dance Festival to Street DanceCompetition,which are well received by both students and teachers.So if you're interested,please write back soon.I'm looking forward to your reply.Yours sincerely,Li Hua第二节One possible version:And then my phone rang again and this time the caller was a man."Are you looking for a dognamed Lola?"he asked.When I said yes,he said:"I think I've got your dog.My daughter foundher in our garden.If you have time,come on over and get her."Hearing the news,I was veryexcited.After hanging up the phone,I told my husband and son the news and we could hardlywait to see our dog again.As I drove to the man's house,I was unsure if I should trust this good news.Turning into hisdriveway,I saw him,smiling,along with his daughter and my dog.Lola looked pretty good."When we found your dog,we knew who to call thanks to the flier in our mailbox.You reallydid a good job,"he said.In order to express my thanks,I offered him some money before I left,but he refused.As I drove home with Lola,I said to myself:This is kindness.部分解析

D.insteadC.aloneD.benefits41.A.illegallyB.secretlyC.topicsD.photographs42.A.giftsB.toolsC.letters43.A.ticketsB.booksD.boringB.firstC.oldD.disappear44.A.previousC.survive45.A.followB.dieD.objectingC.contributingB.lyingD.precious46.A.complainingB.badC.criminalD.basic47.A.unreliableB.generalC.stylishD.Lost48.A.antiqueC.Absorbed第二49.A.BuriedB.DressedD.loveC.anxiety50.A.dislikeB.supportD.scientists51.A.soldiersB.reportersC.officersD.asked52.A.understoodB.wonderedC.studiedD.difficulty53.A.effectB.enemyC.desireD.shortagecor54.A.fearB.lifeC.recordD.showedC.lacked55.A.hadB.took第二节（共10小题；每小题1.5分，满分15分）阅读下面短文，在空白处填人1个适当的单词或括号内单词的正确形式。mIn January,a very sick eagle was delivered to the Montana Raptor Center in Billings,Montana,treats injured birds.The eagle was in bad shape and was 57(bare)breathing.Testingshowed it 58 (poison)by lead().The experts at the center thought the bird didn't have a chance56This is not 60 uncommon event.Since 2015,the center has received 132 eagles,and 9959 (survive).percent have had lead in their blood and most of 61 (they)have died.This particular eagle waslucky,though.It took only two months for it to recover.And it was eventually set free.When it was taken to a hillside,the bird was relatively quiet during the ride.But once the truckapproached the hill,it seemed to know something was coming and began moving around the cage in62 (excite).As soon as the door was opened up,it flew out.But once in the air,the 63 (free)eagle kept wheeling slowly above the experts.It did not fly away until their truck disappeared 64the distance."It's amazing to see that it can have a second chance on life,"says Jordan Spyke,operationsmanager for the center."There's not a much 65 (good)feeling than that."第四部分写作（共两节，满分40分）第一节（满分15分）班级英语角正在征集以“Sharing'”为主题的文章。请你根据下图提示写一篇短文，内容包括：1.你的分享经历；2.简单评论。sharing注意：1.写作词数应为80左右；2.请按如下格式在答题卡的相应位置作答。

35.Luigi Lemoncello's story isaccording to the writer.A.scaryB.excitingC.crazy36.We can know thatA.Odder is hurt by humansB.Odder is saved by a sharkC.Odder is going to live in the wild37.Skandar and the Unicorn Thief is mainly aboutA.the power of a heroB.the secrets of unicornsC.the discovery of a real unicorn38.The underlined(画线的)word“evil”probably means“”in Chinese.A.高尚的B.谨慎的C.邪恶的(B)阅读下面短文，根据语篇内容，从方框内所给的选项中选出能填入空白处的最佳选项，使短文意思通顺，并在答题卡上将该项涂黑。选项中有一项为多余项。We all have those moments when bright ideas hit us.39 We believe creativethinking is the falling apple of Newton,but we seldom notice that creative thinking is infact a process.40 Newton watched an apple fall from a tree while sitting in the garden.Hebegan to wonder if the same force that pulled the apple down also kept the moon goingaround the earth.41 He called it“gravity(重力)”.But when we cheer for thegreat discovery,the whole process of creative thinking shouldn't be forgotten.Newton studied math for long to have a better understanding of the world.Healways enjoyed finding new ways to solve problems.42 It also started the train ofthought that continued for the next twenty years.Newton began to examine "gravity"carefully.He did lots of experiments ()Then he studied the results and usedwhat he had learned to do new experiments.In 1687,the greatest scientific workcame out.九，年级英语第7页（共12页）

姓名准考证号山西省2022~2023学年第一学期八年级期末质量监测英语试卷注意事项：1.本试卷分为两部分。全卷共12页，满分100分，考试时间90分钟（听力测试大约20分钟)。2.答题前，考生务必将自己的姓名、准考证号填写在本试卷及答题卡相应的位置。3.答案全部在答题卡上完成，答在本试卷上无效。4.考试结束后，将答题卡交回。听力部分（共20分)一、情景反应（每小题1分，共5分）本题共5个小题，每小题你将听到一组对话。请你从每小题所给的A、B、C三幅图片中，选出与你所听到的信息相关联的一项，并在答题卡上将该项涂黑。1.A.B0002.AB0003.A.BC.八年级英语第1页（共12页）

alongkind where little placeagain you needone50写作指导：四's the first day of the Christmas holiday(圣诞假期六本篇作要求给同学写封邮件邀请她来参加派对。自说明生日派对的时间和地点，并共长第一节today.Gina doesn'eedb go to work,but she gets up如阅读短文，从语篇。主题：人与社金之社会服务与人际沟通方框中选择适such as London Zoo and the Tower of London.⊙子主题：人际关系与交往当的词并用其At 10 am.Gina gets to London.It's h time visiting the city.Shesn't know London very well.Sshe loses her way soon.She walks一般现在时第正确形式填空，一、三人称agstreet for a long time but can't find London Zoo.At this time空使短文通顺、she sees a man.She goes up to him and says,"Excuse me,sir!I'm newhere.Can you tell e ndon Zoo is?The man looksi.说明来信目的一'm writing to invite you to my意思完整。每表达smiles(徵笑)but doesn't say anything.Just as(正当)Gina is about to askbir空少界群缁信pirthday this Saturday and空限填一词，man puts his hand into his bag and takes out a phone.Hehave a party at home.I'll be glad if you can come.Here is每词限用一次。some words.Then he says,I'm sorry.I can't undead(理解yQuthow you come to my home.Take the No.870 bus and then...turns out that the man comes from Japan.He knownglish.And he3.简要总结→Looking forward to your coming!loses his way,too第二节阅读短文，根据语篇要求填空，使短文通顺、意思四完整。每空限填一词。六☆必备词语与句型：Jennifer moves to a new community.She is free today,so she goes fortell,way,bus,post office,across,street,behind语篇a walk around her communityFirst,she walks along Main Street.On this way,she sees a hospital书Let me tell you the way to my home.for pets ()She has two little dogs,only two years old.She can takeYou can take Bus No.870.themthere to see a doctor.Next to the hospital,there is a bank.Go upMain Street tothe7 end and turn right.She sees a bridge.GacBeSShe表There is a post office across the street空bridge and turn right.On the right,she finds a bowling alley龄球馆).She stopst069_go bowling for thirty minutes..There isaMy home is behind the post office.restaurant across from the bowling alley.She has lunch there.The food1S70 so delicious.After lunch,she comes back home.Now,she knows her community well.And she loves her community.A:Excuse me,sir!(71Are there any post offices?near hereOne possible version:Dear Linda.五B:No,there aren't.There is only a small post office along way from here.六I'm writing to invite you to my birthday party thisA:(72)How far is it from hereSaturday.B:It's about 10 kilometers.You need to take a busThis Saturday is my birthday and I will have a party at全home.It will be great fun.I'll be very glad if you canA:(73)Which bus should I take?come.Let me tell you the way to my home.You can takeB:The No.49 bus.表Bus No.870 and get off at the Kangning Street stop.There is a post office across the street.My home is justA:(74)Can you tell me the way to the bus stopbehind the post office.B:Go across Vale Bridge and you'll find the stop on your efI'm looking forward to your coming!Yours,A:(75)Thank you very muchLi Hua假如你是李华，本周六将在家举办生日派对六(birthday party)。请你写一封邮件邀请同学Linda来参加你的派对，并为她指明路线：乘坐870路公共汽书车在康宁街站下车(get off),马路对面有个邮局，你家就在邮局后面。要求：表1.包括所给提示信息，可适当发挥；2.条理清楚，意思连贯，语句通顺，语法正确：3.60词左右，开头结尾已给出，不计入总词数Dear Linda,六I'm writing to invite you to my birthday partythis Saturday.面表达I'm looking forward to your coming!Yours,Li Hua7

3.Because there are so many problems to deal3.He knocked his opponent(对手)down threewith,I can't go to Hainan for my holiday.(totimes in the first round.do作宾语补足语改写句子)4.Anyone travelling without a passport runs theWith so many problems to deal with,I can'trisk of being arrested(逮捕).go to Hainan for my holiday.5.Researchers continue to learn more about the4.Climbing to the top of Mount Everest is a greatmigration(迁移)patterns and habits ofchallenge for many people.(用todo作主语改写dinosaurs.句子)It is a great challenge for many people toclimb to the top of Mount Everest.Ⅱ.根据汉语提示补全下列句子，每空一词。5.His uncle is going to attend the meeting which1.In order to get its valuable ivory,the two menwill be held next week.(用todo作定语改写句子)killed the elephant without mercy(残忍地)，His uncle is going to attend the meeting to2.Under the guidance of the experts,the localbe held next week.government_has taken action_(已经采取行动)to control the spread of the disease.3.I wish you a Merry Christmas.I send my loveand affection for you as well as (a small gift.Ⅲ.用括号内所给动词的适当形式完成下列短文。It's very important 1.to protect(protect)4.It is said that vast amounts ofthe environment.As high school students,we量的)data on Mars have been collected.can do many things 2.to help (help)protect5.Four Peaks is home to(是...的the environment.家园)black bear,deer,mountain lions and otherOne way we can help is by reducing ourenergy consumption.We can do this by usinganimals.energy-efficient appliances and switching offlights and electronics when they're not in use.摩第1版本期报纸其他版块参考答案Another way to help protect our environment isplanting trees.Forests can make it possible for usTOPIC READING1-2DB3.to enjoy (enjoy)fresh air and animals to have同步课堂a place to live in.Finally,we should encourage1.Silk from Chinapeople around us 4.to take (take)action.考2.A trading route across the seaWe must work together 5.to ensure(ensure)3.the west of Chinawe are doing our part.Only through making4.In the Ming Dynasty5.Zheng Hesmall changes in our lives can we make a big安6.the South China Seadifference in helping protect our planet.7.are being revisited8.other important projects89.explore the Arctic10.reach out across the seaPart 3 Using Language Assessing Your Progress第3版【.根据首字母或汉语提示，写出单词的正确形式。完形填空1.The ceremony is really a good chance for us1-5CACBDstudents to express our thanks to our parents6-10 ACDCBand teachers.M eanwhile,it will turn a new考11-15 ACBABpage in our life.阅读理解2.The necklace was one of her most treasured1-4 BCDDpossessions,but she lost it by accident.案阅读填空1-5 DFAGE

Unit 3 Could you please tell me where the restrooms are?BAnna外文素材改编自Cambridge English PrepareWe're staying on a farm in Utah黄石公园There is nearly nothing!It is too quiet.Most countries have beautiful natural places.Every morning,we get up really early andThese places can be mountains,deserts,beachesride the horses.Then we have breakfastand so on.You can see different animals and plantsand do more riding-not exactly my ideathere.Today let's visit the Yellowstone together.of fun!So,today I walked to a smallYellowstone is one of the oldest national(town near the farm and surfed(冲浪)the家的)parks in the world.It is also a specialInternet there!national park with interesting and unusual30 mins agoplants,animals and landscapes(风景).In[此语篇类型山东潍坊、湖南常德等地中考已考查]Yellowstone,men and women called park rangers1.What does Jessie think of the trip to Sydney?(护林员)look after the animals and plants.A.Boring.B.Common.Yellowstone is on a very big,ancientC.Crowded.D.Great.volcano(火山).The volcano is640,000 years old2.Which of the following can be used toand its landscape is amazing.There are largedescribe Frank's French?forests and some trees which are over 200 yearsA.Very good.B.Very poor.old.The Yellowstone River gives the park itsC.Just so-so.D.Perfect.name,but there are many other rivers.3.What does the underlined word“It”refer to?Over 3 million people visit the park everyA.Internet视频讲解year and they come not only to see the hundredsB.A farm.马褐of plants but also to spend time playing.There√C.A small town.are hot-.water fountains(喷泉)that come up outD.A horse.第3题of the ground.Visitors also enjoy the beautiful4.(中考新考法·细节匹配)Match thelakes,where they can swim or fish.persons with the places where they are andThere are many kinds of animals in the park,choose the right answer.such as birds and fishes.There are sheep and①Utaha.Donna视频讲解bears as well.You don't often see bears because②Parisb.JessieG怨they stay in the forests and mountains,but sheep③Sydneyc.Anna√滴often walk down the roads in the park!A.①-c②-a③-b中考新考法题Yellowstone is usually cold and dry,but thereB.①-a②-c③bare sometimes forest fires.A In winter,it isC.①-b②a③-cvery cold.In summer,it is warm during the day,D.①-c②-b③-abut at night it can be below 0C.People need[此考法江西等地中考已考查]warm clothes when they are camping in the park.5.In which part of a newspaper would you1.What does the underlined word“unusual”probably read the passage?mean in Chinese?A.Travel.B.NatureA.美丽的B.茂盛的C.Culture.D.Sports.C.普通的D.罕见的4

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11.下列实验设计或操作能达到目的的是24滴4滴0.1mol/L日0.1mol/LMgC12溶液FcCl3溶液a液笼Fe2mL热水冰水2mol/LNaOH溶液2NO,≥N,O,△H<0A.探究温度对化

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4.下列实验装置或操作正确的是©人性不得其静量点舍器容ABCDNaOH溶液空气H试纸NaCl溶液/FeSO,溶液润湿的铁粉和碳粉红墨水制备氢氧化亚铁食盐水中提取NaCI测量氯水的pH探究铁的析氢腐蚀5

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野左上方，故需要向左上方移动装片将分裂中期的细胞移至视野中央，D错误。11.B碘液遇淀粉变蓝，向马铃薯匀浆中滴加碘液可以检测是否含有淀粉，但不能检测淀粉是否转化为葡萄:模型建构法可用于研究减数分裂中染

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解：(1)证明：方法一在正方形ABCD中，有AC⊥BD,由己知得EF∥AC.EF⊥BD…1分由折叠的性质知：PD⊥PE,PD⊥PF,PE∩PF=P.PD⊥面PEF…2分又EFC面PEF,故PD L

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1、2024高考名校导航冲刺金卷(一)

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